acc 161  (DSMZ)

Journal: Molecular Therapy Oncology

Article Title: Intravenous oncolytic vaccinia expressing transgenes for enhanced safety, inhibition of shedding, imaging, and systemic cancer immunotherapy

doi: 10.1016/j.omton.2026.201153

Figure Lengend Snippet: Oncolytic vaccinia virus expressing HSV-TK.WT and variants: VV HSV-TK.007 replication efficiency in HeLa producer cells and enhanced sensitivity to GCV versus other VV HSV-TK variants ( A) Growth curve of different vaccinia viruses expressing no VV-TK (VV-TK[−]), HSV-TK WT (HSV-TK.WT), HSV-TK SR39 variant (HSV-TK.SR), HSV-TK dm30 variant (HSV-TK.dm), or HSV-TK TK.007 variant (HSV-TK.007). HeLa cells were infected at a multiplicity of infection (MOI) of 3 with the above viruses. Infected cells were harvested at different times post-infection, and cell lysates were titered using plaque assays. PFU per cell values were determined in three biological replicates. Bars indicate standard deviation (SD). Asterisks indicate statistical significance of PFU per cell of each tested virus at each time point against the control virus (VV-TK[−]), determined by two-way ANOVA followed by Tukey’s multiple comparisons test, ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (B) Replication of vaccinia viruses expressing HSV-TK variants is susceptible to increasing concentrations of GCV. HeLa cells were infected at MOI of 3 with different vaccinia viruses expressing HSV-TK.WT, HSV-TK variants, or no HSV-TK (VV-TK[−]) in the presence of different concentrations of GCV. Twenty-four hours post-infection, infected cells were harvested, and virus titers were determined by plaque assay. PFU per cell values were determined in 3 biological replicates. Bars indicate SD. Asterisks indicate statistical significance against the control virus, determined by two-way ANOVA followed by Dunnett’s multiple comparisons test: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: HeLa (human cervical adenocarcinoma, American Type Culture Collection [ATCC], Manassas, VA, CCL-2), HeLa S3 (human cervical adenocarcinoma, Deutsche Sammlung von Mikroorganismen und Zellkulturen [DSMZ], Braunschweig, Germany, ACC 161), U-2 OS (human osteosarcoma, ATCC HTB-96), VeroB4 ( Cercopithecus aethiops normal kidney cell line, DSMZ ACC 33), and BSC40 (African green monkey kidney cell line, ATCC CRL-2761) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS, Gibco, Grand Island, NY).

Techniques: Virus, Expressing, Variant Assay, Infection, Standard Deviation, Control, Plaque Assay

Journal: Analytical and Bioanalytical Chemistry

Article Title: Comprehensive lipid structure annotation via photochemical epoxidation and mass spectrometry

doi: 10.1007/s00216-025-05953-6

Figure Lengend Snippet: Shotgun MS results for [PC 36:0;Ep + Na] + for A H9c2 and B HeLa cells revealing sn -isomer diagnostic fragment ions. C Comparison of the intensity ( I ) of diagnostic fragments of a sn -isomer divided by the sum of the intensities of both sn -isomers. Mean values and error bars are from three biological replicates, n.d. indicates not detected sn -isomers, dl indicates “detected-in-low-abundance,” and bars with only one sn -isomer identified do not have error bars

Article Snippet: The human cervical HeLa S3 (ACC 161, DSMZ, Germany) and rat H9c2 myoblast cells (Sigma Aldrich, Germany, RRID: CVCL_0286) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, PAN Biotech) with added 10% fetal bovine serum (Sigma Aldrich) as well as 1% 10,000 U/mL penicillin and streptomycin.

Techniques: Diagnostic Assay, Comparison

Journal: Scientific reports

Article Title: Highly specific Immunoproteasome inhibitor M3258 induces proteotoxic stress and apoptosis in KMT2A::AFF1 driven acute lymphoblastic leukemia.

doi: 10.1038/s41598-025-01657-0

Figure Lengend Snippet: Fig. 1. M3258, a highly specific inhibitor of β5i sites, induces apoptosis in ALL cells. (a) Inhibition of proteasome active sites was measured in extracts of RS4;11 and HeLa cells, and in the purified constitutive 26 S proteasomes; n = 2. (b, c) Cells were treated with Btz (b) or M3258 (c) for 48 h, and the viability was assessed by Alamar Blue; n = 2–4. (d) RS4;11 cells were treated with 75 nM and 300 nM M3258 for indicated times, and percentage of apoptotic cells was determined by flow cytometry; n = 2. (e) Cells were treated with M3258 for 12 h, and apoptosis was determined by flow cytometry. In a parallel experiment, RS4;11 cells were treated with M3258 for 4 h, and the chymotrypsin-like (β5) activity was measured by the Proteasome-Glo assay; n = 2. (f) The chymotrypsin-like (β5) activity of the proteasomes in cells treated with M3258 for times indicated was determined by the Proteasome-Glo assay; n = 2. (g) MM1.S cells were pre-treated with IFNγ for three days and then treated with M3258 for 48 h. Viability was measured with Alamar Blue; n = 2. Data on all panels are averages of n biological replicates, and the error bars indicate standard error of the mean.

Article Snippet: Cell lines and cell culture experiments HeLa S3 (RRID: CVCL_0058), RS4;11 (RRID: CVCL_0093), REH (RRID: CVCL_1650), and NALM-6 (RRID: CVCL_0092) cells were obtained from the American Tissue Culture collection; SEM (RRID: CVCL_0095) cells were obtained from DSMZ (Braunschweig, Germany).

Techniques: Inhibition, Purification, Flow Cytometry, Activity Assay, Glo Assay